英文全稱: Recombinant Mouse Stem Cell Factor/SCF/c-Kit Ligand |
縮寫:Stem Cell Factor/SCF/c-Kit Ligand |
種屬:Mouse |
儲(chǔ)存條件:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
中文譯名(僅供參考):干細(xì)胞因子 |
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重組小鼠Stem Cell Factor蛋白 Recombinant Mouse Stem Cell Factor/SCF/c-Kit Ligand |
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細(xì)胞因子檢測 細(xì)胞因子檢測有助于了解其在免疫調(diào)節(jié)中作用, 鑒定分離淋巴細(xì)胞, 監(jiān)測某 些疾病狀態(tài)細(xì)胞免疫功能。例如, 根據(jù)培養(yǎng) CD4+T 細(xì)胞分泌細(xì)胞因子確定細(xì)胞 亞群, 產(chǎn)生 IL - 12 和 IFN -γ者為 Th1 細(xì)胞, 產(chǎn)生 IL - 4 和 IL - 10 者為 Th2 細(xì)胞; 艾滋 病患者 IL - 2 水平明顯降低, 而類風(fēng)濕性關(guān)節(jié)炎、多發(fā)性硬化、移植排斥反應(yīng)等患者 IL - 2水平則升高。細(xì)胞因子檢測方法可分以下 3 種: (1) ELISA。用細(xì)胞因子單克隆抗體包被固相雙抗體夾心法或 ELISPOT 測定法檢 測細(xì)胞因子, 例如檢測 IL - 2, IL - 5, IL - 6。亦可用熒光素標(biāo)記細(xì)胞因子抗體, 對(duì)胞 內(nèi)細(xì)胞因子進(jìn)行染色, 直接以流式細(xì)胞儀測定產(chǎn)生該細(xì)胞因子細(xì)胞數(shù)目。 (2) 生物活性測定法。可根據(jù)細(xì)胞因子生物活性, 選用相應(yīng)實(shí)驗(yàn)系統(tǒng), 包括細(xì) 胞增殖法、直接殺傷法、保護(hù)細(xì)胞免受病毒致病變法等。如選用細(xì)胞因子依賴細(xì)胞 株, 其增殖反應(yīng)與細(xì)胞因子量成正相關(guān), 根據(jù)細(xì)胞株增殖水平可確定樣品中細(xì)胞因 子含量, 如檢測 IL - 1, IL - 2, IL - 4, IL - 6。 (3) 聚合酶鏈反應(yīng) (PCR)。根據(jù)細(xì)胞因子核苷酸序列, 設(shè)計(jì)特定細(xì)胞因子引 物, 利用逆轉(zhuǎn)錄 PCR 測定待檢細(xì)胞中特異 mRNA。該法可用于多種細(xì)胞因子測定。 |
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